Taq DNA polymerase is a highly thermostable enzyme isolated from hot spring bacterium T. aqueticus. It is catalysing 5’→3’ DNA synthesis while not showing 3’→5’ exonuclease proofreading activity. The enzyme works optimally at 72oC and can withstand up to 92oC temperature without compromising structural integrity. The enzyme requires KCl and Magnesium ion for its optimal performance however higher concentration of both ions create mismatch pairing of nucleotides.
Taq DNA polymerase is a highly thermostable enzyme isolated from hot spring bacterium T. aqueticus. It is catalysing 5’→3’ DNA synthesis while not showing 3’→5’ exonuclease proofreading activity. The enzyme works optimally at 72oC and can withstand up to 92oC temperature without compromising structural integrity. The enzyme requires KCl and Magnesium ion for its optimal performance however higher concentration of both ions create mismatch pairing of nucleotides.